neurons with Sindbis viral vectors encoding either GFP or RFP. For isolating infections to one compartment, virus was added to 50 μl taken from the selected compartment, gently mixed, and then added back into the same compartment. Media within the compartment was recirculated three times to ensure proper mixing. The chambers were used 12 to 24 h after infection. Only chambers cultured longer than 14 days were used.
