and brain tissues were collected at P14 (Supplementary Fig. 7k). Cy3-tagged ASO showed high efficiency in the forebrain (Supplementary Fig. 7l), and Sox11 was efficiently knocked down by ASO (Supplementary Fig. 7m). The MBP intensity was remarkably increased in the corpus callosum of Sox11 ASO injected Setdb1 mutant mice (Supplementary Fig. 7n) Sox11 is widely expressed in the neurons as well. To exclude the influence by neurons with deficient Sox11, OL-specific Sox11 knockout mice were generated. For unknown reasons, the Setdb1/Sox11 double mutant mice exhibited severe developmental defects. Nevertheless, the number of CC1+ OLs and the myelinated fibers was significantly increased in Olig1-Cre; Setdb1F/+; Sox11F/F mice compared with that in Setdb1 heterozygous mice (Fig. 6j-l). Myelination defects caused by Setdb1 deficiency was remarkably rescued by suppressing Sox11, highlighting the importance of the OLIG2-SETDB1-SOX11 axis in the myelinogenesis.
