These recent findings underscore the fact that the fundamental mechanism of ATP-dependent chromatin remodeling complexes remains, in large part, unclear and that new techniques must be developed for the assessment of the contributions of long-range topologic effects, the complexity of posttranscriptional histone modifications, and the tissue-specific chromatin states, to name a few of the critical aspects of chromatin that cannot be detected using previously standard in vitro chromatin remodeling methods.
