analyzed for differences in expression by using the average IBAQ values of the 3 technical replicates per line. This revealed the abundant presence (high IBAQ values) of presynaptic and growth cone proteins (Fig 6C) such as Synaptophysin, SNAP25 and Neuromodulin. Glial proteins such as GFAP, ALDH1L1, GLAST, O4 and PDGFaR were not detectable (S1 Table), which indicates that by indirect contact co-cultures one can generate pure neuronal cell cultures. Taken together, these results indicate little variation in protein expression of whole cell lysates between independent experiments and confirmed absence of glial cells in the neuronal cultures.
