A total of 9 samples (Undifferentiated cells without treatment, differentiated cells without treatment, and differentiated cells with alcohol treatment, n=3 for each) were used for MeDIP analysis. Genomic DNA was extracted from the fresh cells immediately after the culture by using a DNeasy blood and tissue kit (Qiagen, Fremont, CA). Briefly, approximately 5×106 cells from each sample were centrifuged to a cell pellet, resuspended in PBS and lysed with Proteinase K. After lysis, DNA was precipitated with 100% ethanol, washed with buffers and eluted in an elution buffer according to the manufacturer's instructions. The DNA quality and quantity was assessed using a Nanodrop spectrophotometer with A260 ratio >1.7 and A230>1.6 considered to be a criteria for quality control. Approximately, 1.5 μg of genomic DNA in 150μl of the buffer from each sample was sonicated using a Branson sonifier to obtain fragment sizes between 200-1000 bp (verified on 2% agarose gel). A 25 μl of sonicated DNA from each sample was kept as Input DNA and the rest of the 100μl was used for Immunoprecipitation with 5-methylcytosine antibody using a Methyl
