On average, 52.5% of reads generated with the OneTouch 100 bp kit had no observed homopolymer errors. Using the 200 bp manual kit, the average percentage of error-free reads drops to 8.3%, but this is markedly improved by using the 200 bp OneTouch kit, with ∼22.2% of reads, on average, free of homopolymer errors (Figure S7). Both 200 bp kits had a lower mean percentage of error-free reads when run on the 316 chip (4.4%–200 bp manual, 17.9%–200 bp OneTouch) versus the 314 (11.1%–200 bp manual, 26.7%–200 bp OneTouch). This difference suggests that even though only the size of the chip was varied, global well accuracy appears to be compromised by higher well densities. The 99th percentile for the number of errors per read was between 11–14 for the 100 bp kit, 33–38 for the 200 bp manual kit and 32–38 for the 200 bp OneTouch kit.
