Nkx2.1-CreER, Pv-ires-Cre animals were bred separately to Ai14 reporter to label CHC and PVBC in the cortex respectively. CHCs were enriched in frontal cortex with tamoxifen induction at E17.5. Intersectional labeling of PCPs were achieved by breeding (a) Sst-Flp, Nos1-CreER for LPN, (b) Sst-Flp, CR-Cre, for MNC (c) VIP-Flp, CR-Cre for ISC and (d) VIP-Flp, CCK-Cre for CCKC separately to Ai65 intersectional reporter that will label cells with tdTomato only when both the lox-Stop-lox and Frt-STOP-Frt cassettes are excised (Figure S1). For validation studies mice were also bred to Nkx-ires-FlpO (He et. al. 2016) to get Nkx2.1-Flp; Cck-Cre; Ai65. Ptn-CreER animals were generated as described in figure S6B and were induced postnatal at P5. Mice were bred and maintained according to animal husbandry protocols at Cold Spring Harbor Laboratory (Institutional Animal Care and Use Committee reference number 16-13-09-8) with access to food and water ad libitum and 12 h light-dark cycle.
