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Chunk #47 — Methods — Genotyping—UW

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Identification, replication, and functional fine-mapping of expression quantitative trait loci in primary human liver tissue.
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Genotyping was performed on each liver sample using the Illumina HumanHap550 (GPL6981) Beadchip platform. Genotyping calls were made using GenomeStudio. After raw genotyping data were loaded into the software, pre-defined cluster definitions were applied and genotype calls were determined. Clusters were checked for separation, deviation from HWE, and lack of variation (i.e., monomorphic). Poorly assigned clusters were modified manually and sites were re-called with corrected cluster definitions. All samples had call rates greater than 98%.