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Chunk #57 — Methods — CFU-S12 assays

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Alcohol and endogenous aldehydes damage chromosomes and mutate stem cells.
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To assess the survival of CFU-S12 after exposure to acetaldehyde, we treated total bone marrow cells with 4 mM acetaldehyde for 4 h in vitro before injecting them into lethally irradiated recipient mice. After 12 days, the number of CFU-S were counted. Survival was expressed relative to the untreated control for each genotype. Each data point represents the mean CFU-S survival in ten recipient mice, expressed relative to untreated samples of the corresponding genotype.