Ara-C (2%, Sigma) in vehicle (0.9% saline) or vehicle alonewere infused onto the surface of the brain of adult mice (coordinates: anterioposterior, 0, lateral, 1.1, dorsoventral, 1.5mm medial to lateral relative to bregma) for 6 days with micro-osmotic pumps (Alzet, model 1007) as previously described by Doetsch et al. (1999). EGF (100nm/ul, Upstate) in vehicle (0.9% saline), or vehicle alone was infused into the LV in adult GFAP-GFP and WT mice (infusion coordinates: anterioposterior, 0; lateral, 1.1; dorsoventral, 1.5 mm medial to lateral relative to bregma) for 5 days using micro-osmotic pumps (Alzet, model 1007). Mice were then processed for FACS-purification, cell culture or histology.
