processing. Following antigen retrieval (Citrate Buffer, 20 min, 90 °C), tissue sections were blocked for 2 h at RT in 0.3% Triton-X in PBS, containing 5% normal donkey serum. The sections were incubated overnight at 4 °C with the primary antibodies (mouse anti-CD83, HB15a, Beckman Coulter, and polyclonal goat anti-Iba1, NB100–1028, Novus Biologicals, both diluted 1:250), followed by staining with secondary antibodies (donkey-anti-mouse-AlexaFluor488, Jackson Immunoresearch, 1:250, and donkey-anti-goat-AlexaFluor568, Invitrogen, 1:250) and DAPI for 2 h at RT. The stained sections were subsequently mounted onto glass slides and mounted using the aqueous mounting medium Faramount (Agilent Dako, UK) under glass coverslips.
