Genomic DNA was purified from whole blood using Puregene chemistry on the Qiagen Autopure LS according to standard automated Qiagen protocols (Valencia, CA). DNA samples were quantitated via the ND-8000 spectrophotometer and DNA quality was evaluated via gel electrophoresis on a 0.8% agarose gel. The concentration for all qualified samples was normalized to 50 ng/ul and samples were arrayed in Matrix 0.5ml 2D barcoded tubes in racks of 96. Sample identity was confirmed by genotyping 8 SNPs using Taqman allelic discrimination assays (Applied Biosystems; CA) and assessing for concordance with historical data.
