In the second phase, blood and urine specimens were collected for comprehensive laboratory testing and a physician and nurse practitioner performed a medical history and physical examination. Intima-media thickness was assessed by carotid Doppler. Electrocardiography was performed, and measures of pulse wave velocity were obtained. Bone mineral density and body composition were measured by dual-energy X-ray absorptiometry. Physical functioning was assessed by grip strength and a lower extremity function test. Nutrition was measured by a second 24-hr dietary recall using the AMPM. A battery of neuropsychological tests assessed cognitive performance, and an audio computer-assisted survey program administered psychosocial inventories. The audio-administered questionnaires included inventories on ethnic identity, income assessment, social support, and psychiatric screening coping strategies. Further information about study measure can be accessed at the HANDLS Web site, http://handls.nih.gov.
