Chunk #12 — Methods — Fluorescent in situ hybridization (FISH)

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Alcohol reverses the effects of KCNJ6 (GIRK2) noncoding variants on excitability of human glutamatergic neurons.

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Fluorescent, single molecule in situ hybridization was performed with the RNAscope® Multiplex Fluorescent Detection Kit v2 (Advanced Cell Diagnostics, ACD) following the manufacturer’s instructions and details provided in the Supplementary Methods.