Human iPSC-derived neurons were generated from iPSC bulk cultures as previously described6. Our differentiation and activation protocol matched the protocol recently described by Hook et al.34, where they showed robust KCl-induced neurotransmitter release further strengthening the assertion that our neurons are relevant for the investigation into endogenous human neuron function. IPS cell lines used were H9, C11 and C32. C11 iPSCs were derived from male fibroblasts (ATCC CRL2429) and C32 iPSCs from female fibroblasts (ATCC CRL1502). All iPSC experiments used 3 technical replicates from each of these 3 cell lines. Primer sequences for quantitative PCR analysis are listed in Supplementary Table 2 and statistics were determined using an unpaired t-test (n > 3).
