paperKB
coga / coga-kb
Help
Sign in

Chunk #13 — Materials and methods — Independent RNA-seq data used for validating elastic net-derived splicing models

Source
RNA alternative splicing impacts the risk for alcohol use disorder.
Embedded
yes

Text

We used genotype and RNA-seq data from 139 human postmortem brain samples of the superior frontal gyrus (Brodmann area 8) that were previously reported [31]. PSI of the modeled events were quantified using rMATS with Gencode annotation (GRCh37.p13, hg19). To compare the analysis from this cohort with CMC, the same standards were imposed. Skipped exons that have: (i) all marker variants available in the genotypes of the PFC samples; (ii) more than 100 samples with ≥10 junction reads; and (iii) PSI with IQR > 0.1, were compared.