All Drosophila strains were outcrossed for 5 to 10 generations to the Berlin genetic background containing the eye color marker mutation w1118 (Berlin). Flies were maintained on standard cornmeal/molasses/yeast media at 25°C and 70% relative humidity with an approximately 16/8 h light/dark schedule. Strains used in this study were obtained from the Bloomington Stock Center, Harvard University, the Drosophila Genetics Resource Center (Japan), the National Institute of Genetics (Japan), the Szeged Stock Center (Hungary), Petros Ligoxygakis (d03636), and Leslie Vosshall (Or83b1, Or83b2). Insertion lines (4.43, 5.10, 10.219, 11.247, 11.83) of the transposon P{GawB} were generated in the Heberlein lab.
