DNA extracted from two cancer cell lines (HCC38, HCC1143) was mixed with DNA from matched B-lymphocyte cell lines (HCC38BL, HCC1143BL) in various proportions, and hybridized to Affymetrix 250 K Sty SNP arrays. Stock DNA aliquots were created for each cell-line by normalization of DNA concentration to 50ng/μl. Mixing of cancer and matched B-lymphocyte DNA to each required mixing fraction was done by volume.
