Lumbar puncture was performed between the L3/L4 or the L4/L5 intervertebral space, and the subsequent CSF was collected in polypropylene tubes. The analyses included routine parameters and the core AD biomarkers, i.e., Aβ42, T-tau, and P-tau. The AD biomarkers were quantified with sandwich ELISAs [INNOTEST amyloid-β42, hTau, and Phospho-Tau (181P), respectively; Fujirebio Europe, Ghent, Belgium]. The AD biomarker analyses from both clinics were all carried out at one central laboratory.
