Chunk #6 — Materials and methods — Antibodies

Source

Developmental regulation of G protein-gated inwardly-rectifying K+ (GIRK/Kir3) channel subunits in the brain.

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Rabbit anti-GIRK1 polyclonal, guinea pig anti-GIRK2 polyclonal and rabbit anti-GIRK3 polyclonal antibodies (Aguado et al., 2008) were used in this study. The detailed characterization of the antibodies targeting GIRK channel subunits has been described elsewhere (Aguado et al., 2008; Fernández-Alacid et al., 2009).