centrifuged at 130 g for five minutes. The cell pellet was then resuspended in Dulbecco’s phosphate-buffered saline (DPBS, Invitrogen, 14287) containing 0.02% BSA and 12.5U/ml DNase and filtered through a 20 μm Nitex mesh (Sefar America Inc., Lab Pak 03-20/14) to remove undissociated cell clumps. Cell health was assessed by trypan blue exclusion. Only single cell suspensions with > 85% viability were used for experiments.
