of further exploration. TNFSF11 was negatively correlated with ASB16-AS1. RANKL encoded by TNFSF11 is a key factor for osteoclast differentiation and activation [54]. Taken together, we predicted that ASB16-AS1 may play important roles in osteoblast and osteoclast proliferation and differentiation. BMP2 has been shown to potently induce osteoblast differentiation [55]. ALPL, coding for the liver/bone/kidney isozyme of alkaline phosphatase, are known to be regulated during osteoblastic differentiation [56]. The expression of BMP2 and ALPL were increased after transfection of pCEP4-ASB16-AS1, suggesting that ASB16-AS1 may promote the differentiation of osteoblast.
