500ng of total RNA was hybridized for 17 hours using the lincRNA codeset. The hybridized material was loaded into the nCounter prep station followed by quantification on the nCounter Digital Analyzer following the manufacturer’s protocol. For RNA immunoprecipitation experiments, we used a modified protocol. After reverse crosslinking, RNA was extracted using phenol/chloroform and ethanol precipitation methods and resuspended in 10ul of H2O. 5ul of the eluted material was hybridized for 17 hours using the lincRNA codeset.
