We took measures every minute before drug superfusion (control) and during drug superfusion. All values are expressed as mean ± s.e.m. We performed statistical analysis using GraphPad Prism version 5.00 (GraphPad Software, San Diego California USA). In the case of a single drug application, we used Student’s paired t-test. One-way repeated measures ANOVA with the Tukey post hoc test were performed in a multiple drug application. Statistical significance was set at p < 0.05.