cell-type composition by restricting our analysis to AD brains. We observed a significant association in the multi-area analysis of the MSBB dataset (β = − 0.03 p = 4.01 × 10−02; Table 4; Fig. 4b; Additional file 1: Table S11; Additional file 1: Figure S9b) and also a significant increase in relative proportion of astrocytes (β = 0.03; p = 1.23 × 10−02; Table 4; Fig. 4b; Additional file 1: Table S11; Additional file 1: Figure S9b). We also observed a significant decrease in relative proportion of neurons (β = − 0.06; p = 2.11 × 10−02; Table 4; Fig. 4c) when we analyzed the LOAD and control brains from the Knight-ADRC. When we restricted the analysis to AD brains from the Knight-ADRC and compared the APOE ε4 carriers (n = 44) to non-carriers (n = 40) we also observed a decreased relative neuronal proportion (β = − 0.06; p = 2.69 × 10−02; Table 4; Fig. 4d). We extended the models to correct for the Braak stages and observed a significant association for the relative proportion of neurons with the APOE ε4 allele in the Knight-ADRC dataset (β = − 0.06; p = 3.66 × 10−02; Table 4) and
