Ex vivo levels of anandamide in the BLA were significantly elevated in mice that had received CS–US training, relative to CS-alone training (ANOVA effect of training: F1,38 = 7.03, P<0.05) (Figure 2b). In addition, AM3506 treatment increased anandamide levels in both training groups, relative to vehicle (ANOVA effect of treatment: F1,38 = 8.69, P<0.01, n = 10–11). AM3506-treated mice also increased anandamide levels in the ventromedial prefrontal cortex (ANOVA effect of treatment: F1,43 = 15.76, P<0.01, n = 10–13) and the dorsal striatum (ANOVA effect of treatment: F1,46 = 49.82, P<0.01, n = 11–15) (Figure 2c), relative to vehicle controls but, in contrast to BLA, neither region showed a significant increase as a result of CS–US training.
