or stably expressed (Corcoran et al., 2008; Krut et al., 2006). The nSMase3 protein is a C-tail-anchored integral membrane protein containing an ER signal in the C-terminus and a proline-rich region at the N-terminus, which could potentially mediate protein-protein interactions (Fig. 1) (Corcoran et al., 2008). Northern blot analysis revealed that nSMase3 mRNA was ubiquitously expressed as a 4.6-kb mRNA with the highest expression in striated muscle and heart muscle. Finally, confocal microscopy studies reported localization of nSMase3 to both the ER and Golgi.
