kb relative TSS, t13=2.49, p=0.02, Fig. 2h and Supplementary Fig. 5b,e). Enrichment of H3K9me2 showed no difference between the rat strains at any examined loci except +0.17 kb downstream the TSS in the AcbSh (t14=3.31, p=0.005, Supplementary Fig. 5c,f). These region-specific epigenetic changes are in line with the transcriptional differences observed between the strains. Concomitant with the histone post-translation modifications, SHRs exhibited greater cytosine methylation immediately upstream the TSS within a conserved CpG island (t16=2.60, p=0.019), but not within the gene-body (t18=0.74, p=0.47, Fig. 2i).
