Luminex® flow cytometry-based technology allows for simultaneous quantification of proteins in a single multiplex assay, thus providing an advantage of increased throughput and decreased volume needed per sample. This is achieved via fluorescent-coded magnetic polystyrene microspheres (80;6.45μm) which are coated with specific capture antibodies that allow multiple proteins to be measured via sandwich ELISAs in a single sample. Using the Luminex® 200 platform, our lab used the MILLIPLEX® MAP Human Amyloid Beta and Tau Panel (Kit # HNABTMAG-68K) to quantify Aβ40, Aβ42, Total Tau proteins (Tau), and Phosphorylated Tau Thr181 (pTau) following manufacturer protocol (Luminex® Corp, Austin TX; EMD Millipore Corporation, Billerica, MA).
