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Chunk #44 — EXPERIMENTAL PROCEDURES — Identification of DNA methylation probes for imprinted genes

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Recurrent variations in DNA methylation in human pluripotent stem cells and their differentiated derivatives.
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were slightly different for the 450K DNA Methylation array data. CpG probes were annotated to imprinted genes using the 450K DNA Methylation array manifest file, or if it fell within 5 kb upstream of the gene according to UCSC hg18. All imprinted probes were partially methylated (0.20<β<0.80) in at least 90% of tissue samples. Maternal imprints were unmethylated (β<0.20) in at least 2 androgenetic samples and fully methylated (β>0.80) in at least 2 gynogenetic samples. Paternal Imprints were unmethylated (β<0.20) in at least 2 gynogenetic samples and fully methylated (β>0.80) in at least 2 androgenetic samples (Table S5B).