Mothers fed dsRNA were dissected in egg buffer (118 mM And, 40 mM KCl, 3 mM CaCl2, 3 mM MgCl2, 5 mM HEPES pH 7.2) on a coverslip to release young embryos. The cover-slip was inverted onto a 3% agar pad, which was then sealed with petroleum jelly. A series of 12 focal planes was recorded every 30 sec for 1 h using Openlab software (Improvision) controlling either a Zeiss Axioplan 2 or Leica DMBRE microscope.
