We performed S-LDSC (v.1.0.1)32 to evaluate global ancestry-associated DEGs for their enrichment for heritability of complex traits, mainly focusing on 17 brain and five immune-related traits as a positive control. We downloaded GWAS summary statistics of each trait from the sources listed in Supplementary Data 13. Following recommendations from the LDSC resource website (alkesgroup.broadinstitute.org/LDSCORE/), we ran S-LDSC for each list of candidate genes. We used the baseline LD model (v.2.2), which included 97 annotations, to control for the LD between variants with other functional annotations in the genome. To remove other potential confounding factors in our analysis, we also included one annotation of all protein-coding genes.
