Chunk #35 — PROCEDURE — Preparation of sgRNA expression construct

Source

Genome engineering using the CRISPR-Cas9 system.

Embedded

yes

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? TROUBLESHOOTING viPurify the PCR product by using the QIAquick PCR purification kit according to the manufacturer's directions. Elute the DNA in 35 μl of EB buffer (part of the kit) or water.