Inter-individual variability in IFN-β responsea) t-SNE plot of unstimulated (blue) and IFN-β-stimulated (red) PBMCs and the estimated cell types. b) Cell type-specific expression in stimulated (left) and unstimulated (right) cells. Differentially expressed genes shown (FDR < 0.05, |log(FC)| > 1). Each column represents cell type-specific expression for each individual from demuxlet. c) Observed variance (y-axis) in mean expression over all PBMCs from each of the 8 individuals versus expected variance (x-axis) over synthetic replicates sampled across all cells (light blue, pink) or replicates matched for cell type proportion (blue, red). d) Cell type proportions for each individual in unstimulated and stimulated cells. e) Correlation between sample replicates in control and stimulated cells. f) Number of significantly variable genes in each cell type and condition.
