Adult neural progenitors were isolated from adult mice hippocampi (C57BL/6) and cultured as monolayer on plastic dishes in DMEM/F12 medium supplemented with N2, FGF-2 (20 ng/ml), heparin (5 μg/ml) and EGF (20 ng/ml) as previously described (Ma et al., 2008). Neuronal differentiation of these adult neural progenitors was induced by withdrawal of growth factors and treatment of retinoic acid (0.5 μM), forskolin (1 μM) and fetal bovine serum (0.5%) and cultures were continued for 2 to 12 days.
