In fact, more results generated using in vitro brain slices show a stronger effect of EtOH on GABA release, as discussed earlier in this review (Fig. 1). In vitro brain slice preparations provide a number of highly sensitive experimental strategies that can be employed to detect presynaptic changes in transmitter release (for reviews of these approaches, see Siggins et al. 2005; Weiner and Valenzuela 2006).
