To optimize cellular labeling for CART and KISS1, mice were injected ICV with 5μL of sterile colchicine (2mg/mL), as described previously 45. Mice were anesthetized with ketamine/xylazine and mounted in a stereotaxic frame. A 33 guage cannula was placed into the lateral cerebral ventricle using the coordinates: +0.3mm anterior-posterior to bregma, +0.8mm lateral to the midline, −2.5mm dorsal-ventral. Colchicine was injected over a 15min period through the cannula using a hamilton syringe. Following injection the cannula was removed and the surgical incision was closed with wound clips. Mice were anesthetized and transcardially perfused for brain immunohistochemistry 36h after colchicine treatment.
