2. Numbers of individuals per pool and numbers of pools: To obtain maximal benefits from multiple pool genome wide association: 1) The numbers of individuals in each pool should be sufficient that even sophisticated analyses of pooled data cannot reconstruct individual identities or genotypes. Treatments of this subject suggest that pools need to contain more than 4–5 individuals for maximal confidentiality protection [125]. 2) The numbers of individuals in each pool should allow significant cost/time savings compared to individual genotyping. 3) The numbers of pools should be sufficient to provide good estimates of pool-to-pool variability, which can then be used to compare to the differences between disease and control individuals using standard statistical tests.
