treatments, cells were collected in 200 μl of Passive Lysis Buffer and stored at −80°C at least overnight to allow for cell membrane disruption. Cell lysates were diluted in Passive Lysis Buffer and each sample was quantified in triplicate on Perkin-Elmer Victor3 V plate reader. The sequential addition of Luciferase Assay Reagent II and Stop & Glo reagent allowed for the measurement of firefly and Renilla luciferase activity, respectively.
