Sections were mounted onto glass slides with FluorSave Reagent (Calbiochem, USA). All the images were acquired using the same settings under a Leica TCS-SP2-AOBA confocal laser-scanning microscope (Leica Microsystems, Heidelberg GmbH, Mannheim, Germany) employing the 63× N.A. 1.4 oil objective. All the confocal images were acquired with the same settings and fluorescence distribution was analyzed by the Leica Confocal Software “Leica Lite,” version 2.61. Graphs represent the number of cellular co-localizations expressed as a percentage (%) of mitochondria co-localized with the NLRP3 receptor or the NLRP3 receptor with caspase-1 activity after the different treatments had been applied.
