Preparation of iPSC from human primary lymphocytes using Sendai viral vectors (CytoTune™, Life Technologies) has been described24. All biomaterials were de-identified repository specimens and therefore are exempt from human subjects regulations. Midbrain-like DA neurons were generated using a dual SMAD inhibition protocol26. Excitatory neurons were induced through a modified protocol27 where during day 5 of the protocol iNs were gently dissociated with accutase (StemCell Technologies), and (7.0 × 104) were co-cultured with mouse glia (3.5 × 104 cells)54. Electrophysiological analyses were performed following at least 30 days of differentiation and maturation. Additional details are found in the Supplemental Methods.
