Transverse hippocampal slices were prepared as previously described (Kleschevnikov et al., 2012a; Salehi et al., 2009). In short, a mouse was anesthetized with isoflurane before decapitation. The brain was quickly removed and immersed for 2 min in ice-cold artificial cerebrospinal fluid (ACSF) containing 119 mM NaCl, 2.5 mM KCl, 2.5 mM CaCl2, 1.3 mM MgSO4, 1 mM NaH2PO4, 26 mM NaHCO3, 10 mM glucose, osmolarity 305 mOsm, continuously bubbled with 95% O2-5% CO2, pH 7.4. The hippocampus was dissected and cut into 350-μm-thick slices in ice-cold ACSF with a vibratome (Leica VT1000S). Slices were allowed to recover in oxygenated ACSF at room temperature for at least 2 h prior to experimental recordings.
