with U6 snRNA as an internal control probe. All the eight miRNAs showed significant downregulation in patient-derived neurospheres (Figure 3d). Importantly, downregulation of miR-106b detected in patient neurospheres is also reported in schizophrenic brains36 and Df(16)A+/− mice.9 Complementing these results, pri-miR-106b, the pri-form of miR-106b, was upregulated in patient-derived neurospheres (P=0.0466; Supplementary Figure 3c), suggesting that the processing efficiency of pri-form to pre-form of miR-106b was lowered, probably due to reduced DGCR8 expression.
