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Chunk #27 — Discussion

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Alcohol use disorder causes global changes in splicing in the human brain.
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of human fetuses exposed to alcohol. Qualitatively, eight mis-splicing events were detected: 5’ and 3’ alternative splice sites, mutually exclusive exons, intron retention, cassette exon, coordinate cassette exon, alternative first exon, and alternative last exon (exon skipping was likely not assessed by employed computational pipeline), with intron retention being the most frequent event. Differences between this and our study may possibly be attributed to different developmental stages of the brain (fetus vs adult) as well as to different bioinformatics approaches.