et al., 2021). This can be due to differences in protocol, the particular readout of neural identity used in the previous reports, or thresholds for assigning positive staining from immunohistochemistry (Figure S2). Together, these data suggest that NGN2-iNs generated from our protocol are comparable with other published reports of induced neurons resulting from NGN2 overexpression, and differences in the method of readout (selected markers versus whole transcriptome) can influence the interpretations of heterogeneity.
