Consistent with the clustering observed, CBC showed the greatest number of region-restricted or region-enriched DEX genes with 516 (4.8%) of 10,729 genes spatially DEX (Supplementary Information 6.4; Supplementary Table 5). In contrast, the number of genes highly enriched in the other regions was lower: NCX (46; 0.43%), HIP (48; 0.45%), AMY (4; 0.04%), STR (137; 1.28%), and MD (216; 2.01%). The majority of these spatially enriched genes were also temporally regulated, and some were transiently enriched during a narrow time-window, such as those featured in Supplementary Figures 14 and 15 (NCX: FLJ32063, KCNS1; HIP: CDC20B, METTL7B; AMY: TFAP2D, UTS2D; STR: C10ORF11, PTPN7; MD: CEACAM21, SLC24A5; CBC: ESRRB, ZP2). These clustering and region-enrichment results reveal that regional transcriptomes are developmentally regulated and likely reflect anatomical differences.
