(p=2.02e-3) or hCOs (p=4.44e-4) (bottom panel in Figure 4E). Especially, chromatin regions related to oligodendrocyte genes were specifically open in hMGEOs, and those involved in endothelium development were specifically open in hCOs. Importantly, open chromatin regions unique to hMGEOs were observed in genes critical in MGE development (e.g. NKX6-2, FOXA2, OLIG2 and DLX6), whereas genes involved in cortex development (e.g. PAX6, MGP, GLI3 and EMX2) displayed hCOs-specific open chromatin regions (Figure 4G). Therefore, our results suggest an active role of chromatin accessibility in transcriptional regulation during MGE and cortex development.
