Both male and female mice were used for immuno-histological and biochemical studies, and only male mice for behavioral studies. At weaning (postnatal day 21), pups were separated from their mothers and reared either in social isolation (two mice were separated by a median barrier in one mouse cage) or in social groups (2 to 5 mice per cage). Mice were given access to food and water ad libitum. This experimental protocol lasted until animals were used for behavioral, biochemical, or histological analysis. No obvious differences were detected between sex in histological and biochemical studies.
