Mice aged 9 to 16 weeks were tested first for ethanol-induced loss-of-righting-reflex (LORR) at 3 doses of ethanol (3.2, 3.6, and 4.0 g/kg) in a random-order balanced design with a 1 week interval between each dose. The same mice were used for the 2-bottle-choice ethanol consumption experiment, and were allowed to rest for 2 weeks after the final LORR test. LORR and 2-bottle choice ethanol consumption experiments were performed as described in Kapfhamer and colleagues (2008). Both males and females were tested; we did not detect a significant difference in Lmo3 expression between males and females, so data from males and females were combined in the analysis.
