gene lengths are larger than the RefSeq Genes model (as observed for GABAB1), RPGM can be calculated based on the new gene lengths. We further considered splice junctions in exon, intron, and intergenic regions and defined a calculation referred to as RPJM. Previous research has only identified splice junctions without comparing them quantitatively (27, 30). However, the RPJM strategy introduced here allows quantitative comparisons among experimental groups.
